› 4x2r

X-ray diffraction
1.05Å resolution

Function and Biology Details

Reaction catalysed: 
1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111064 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 25.91 KDa
Source organism: Actinomyces urogenitalis DSM 15434
Expression system: Escherichia coli
UniProt:
  • Canonical: C0W7K4 (Residues: 1-247; Coverage: 100%)
Gene names: HMPREF0058_1848, hisA
Sequence domains: Histidine biosynthesis protein
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand PO4 4 x PO4
Ligand CXS 1 x CXS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41
Unit cell:
a: 76.02Å b: 76.02Å c: 43.179Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.123 0.123 0.141
Expression system: Escherichia coli

Quick links

Citations

1 review citation

How enzyme promiscuity and horizontal gene transfer contribute to metabolic innovation.
Glasner et al. (2020)
10 other articles

2 mentions without citation

Evolution of substrate specificity in a retained enzyme driven by gene loss.
Juárez-Vázquez et al. (2017)
1 more