› 4x4w

X-ray diffraction
1.9Å resolution

Crystal structure of the full-length human mitochondrial CCA-adding enzyme

Released:
DOI: 10.2210/pdb4x4w/pdb
PDB entry 4x4w coloured by chain, front view.
PDB entry 4x4w coloured by chain, side view.
PDB entry 4x4w coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
On-enzyme refolding permits small RNA and tRNA surveillance by the CCA-adding enzyme.
Kuhn CD, Wilusz JE, Zheng Y, Beal PA, Joshua-Tor L
Cell 160 644-658 (2015)
PMID: 25640237

Function and Biology Details

Reaction catalysed: 
(1a) a tRNA precursor + CTP = a tRNA with a 3' cytidine end + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188688 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CCA tRNA nucleotidyltransferase 1, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 416 amino acids
Theoretical weight: 47.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96Q11 (Residues: 27-434; Coverage: 94%)
Gene names: CGI-47, TRNT1
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand SO4 5 x SO4
Ligand FLC 2 x FLC
Ligand GOL 6 x GOL
Ligand CL 8 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 40.97Å b: 142.36Å c: 100.59Å
α: 90° β: 94.64° γ: 90°
R-values:
R R work R free
0.156 0.154 0.196
Expression system: Escherichia coli

Quick links

Citations

19 review citations

tRNA Modifications: Impact on Structure and Thermal Adaptation.
Lorenz et al. (2017)
18 more

3 mentions without citation

On-enzyme refolding permits small RNA and tRNA surveillance by the CCA-adding enzyme.
Kuhn et al. (2015)
2 more