› 4x4x

X-ray diffraction
2.25Å resolution

Retrofitting antibodies with stabilizing mutations. Herceptin scFv mutant.

Released:
DOI: 10.2210/pdb4x4x/pdb
PDB entry 4x4x coloured by chain, front view.
PDB entry 4x4x coloured by chain, side view.
PDB entry 4x4x coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Langley DB, Roome B, Christ D

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213645 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Human Variable Heavy Chain of Herceptin Chains: A, C
Molecule details ›
Chains: A, C
Length: 140 amino acids
Theoretical weight: 14.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
Human Variable Light Chain of Herceptin Chains: B, D
Molecule details ›
Chains: B, D
Length: 107 amino acids
Theoretical weight: 11.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P3121
Unit cell:
a: 91.19Å b: 91.19Å c: 114.35Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.226 0.269
Expression system: Escherichia coli BL21(DE3)

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