› 4x5s

X-ray diffraction
1.95Å resolution

The crystal structure of an alpha carbonic anhydrase from the extremophilic bacterium Sulfurihydrogenibium azorense.

Released:
DOI: 10.2210/pdb4x5s/pdb
PDB entry 4x5s coloured by chain, front view.
PDB entry 4x5s coloured by chain, side view.
PDB entry 4x5s coloured by chain, top view.
Source organism: Sulfurihydrogenibium azorense
Primary publication:
Crystal structure of the most catalytically effective carbonic anhydrase enzyme known, SazCA from the thermophilic bacterium Sulfurihydrogenibium azorense.
De Simone G, Monti SM, Alterio V, Buonanno M, De Luca V, Rossi M, Carginale V, Supuran CT, Capasso C, Di Fiore A
Bioorg Med Chem Lett 25 2002-6 (2015)
PMID: 25817590

Function and Biology Details

Reaction catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111102 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase Chains: A, B
Molecule details ›
Chains: A, B
Length: 232 amino acids
Theoretical weight: 26.65 KDa
Source organism: Sulfurihydrogenibium azorense
Expression system: Escherichia coli
UniProt:
  • Canonical: C1DTU5 (Residues: 27-254; Coverage: 90%)
Gene name: SULAZ_0541
Sequence domains: Eukaryotic-type carbonic anhydrase
Structure domains: Alpha carbonic anhydrase

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand AZM 2 x AZM
Ligand PE8 2 x PE8
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 46.47Å b: 89.43Å c: 114.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 0.214
Expression system: Escherichia coli

Quick links

Citations

10 review citations

An Overview of the Bacterial Carbonic Anhydrases.
Supuran et al. (2017)
9 more

4 mentions without citation

Thermostable Carbonic Anhydrases in Biotechnological Applications.
Di Fiore et al. (2015)
3 more