› 4x6k

X-ray diffraction
1.94Å resolution

Crystal structure of the intramolecular trans-sialidase from Ruminococcus gnavus in complex with Siastatin B

Released:
DOI: 10.2210/pdb4x6k/pdb
PDB entry 4x6k coloured by chain, front view.
PDB entry 4x6k coloured by chain, side view.
PDB entry 4x6k coloured by chain, top view.
Source organism: [Ruminococcus] gnavus CC55_001C
Primary publication:
Discovery of intramolecular trans-sialidases in human gut microbiota suggests novel mechanisms of mucosal adaptation.
Tailford LE, Owen CD, Walshaw J, Crost EH, Hardy-Goddard J, Le Gall G, de Vos WM, Taylor GL, Juge N
Nat Commun 6 7624 (2015)
PMID: 26154892

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165347 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anhydrosialidase Chain: A
Molecule details ›
Chain: A
Length: 486 amino acids
Theoretical weight: 53.73 KDa
Source organism: [Ruminococcus] gnavus CC55_001C
Expression system: Escherichia coli
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ACE 1 x ACE
Ligand 3XR 1 x 3XR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: R3
Unit cell:
a: 99.109Å b: 99.109Å c: 131.708Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.177 0.225
Expression system: Escherichia coli

Quick links

Citations

26 review citations

Introduction to the human gut microbiota.
Thursby et al. (2017)
25 more

2 mentions without citation

Biochemical and structural basis of sialic acid utilization by gut microbes.
Bell et al. (2023)
1 more