› 4xf5

X-ray diffraction
1.45Å resolution

Crystal structure of a TRAP periplasmic solute binding protein from Chromohalobacter salexigens DSM 3043 (Csal_0678), Target EFI-501078, with bound (S)-(+)-2-Amino-1-propanol.

Released:
DOI: 10.2210/pdb4xf5/pdb
PDB entry 4xf5 coloured by chain, front view.
PDB entry 4xf5 coloured by chain, side view.
PDB entry 4xf5 coloured by chain, top view.
Source organism: Chromohalobacter salexigens DSM 3043
Entry authors: Vetting MW, Al Obaidi NF, Toro R, Morisco LL, Benach J, Wasserman SR, Attonito JD, Scott Glenn A, Chamala S, Chowdhury S, Lafleur J, Love J, Seidel RD, Whalen KL, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173021 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Twin-arginine translocation pathway signal Chains: A, B
Molecule details ›
Chains: A, B
Length: 347 amino acids
Theoretical weight: 39.74 KDa
Source organism: Chromohalobacter salexigens DSM 3043
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q1QZR9 (Residues: 27-350; Coverage: 100%)
Gene name: Csal_0678
Sequence domains: Bacterial extracellular solute-binding protein, family 7
Structure domains: Bacterial extracellular solute-binding protein, family 7

Ligands and Environments

2 bound ligands:
Ligand CL 4 x CL
Ligand 2A1 2 x 2A1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2
Unit cell:
a: 122.605Å b: 51.154Å c: 111.943Å
α: 90° β: 115.96° γ: 90°
R-values:
R R work R free
0.165 0.164 0.191
Expression system: Escherichia coli BL21(DE3)

Quick links