› 4xfe

X-ray diffraction
1.4Å resolution

Crystal structure of a TRAP periplasmic solute binding protein from Pseudomonas putida F1 (Pput_1203), Target EFI-500184, with bound D-glucuronate

Released:
DOI: 10.2210/pdb4xfe/pdb
PDB entry 4xfe coloured by chain, front view.
PDB entry 4xfe coloured by chain, side view.
PDB entry 4xfe coloured by chain, top view.
Source organism: Pseudomonas putida ND6
Entry authors: Vetting MW, Al Obaidi NF, Toro R, Morisco LL, Benach J, Wasserman SR, Attonito JD, Scott Glenn A, Chamala S, Chowdhury S, Lafleur J, Love J, Seidel RD, Whalen KL, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125093 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TRAP dicarboxylate transporter subunit DctP Chain: A
Molecule details ›
Chain: A
Length: 341 amino acids
Theoretical weight: 38.72 KDa
Source organism: Pseudomonas putida ND6
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: I3UZ52 (Residues: 1-323; Coverage: 100%)
Gene name: YSA_07416
Sequence domains: Bacterial extracellular solute-binding protein, family 7
Structure domains: Bacterial extracellular solute-binding protein, family 7

Ligands and Environments

3 bound ligands:
Ligand BDP 1 x BDP
Ligand CXS 1 x CXS
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2
Unit cell:
a: 130.001Å b: 35.054Å c: 60.833Å
α: 90° β: 99.54° γ: 90°
R-values:
R R work R free
0.158 0.156 0.186
Expression system: Escherichia coli BL21(DE3)

Quick links