Function and Biology Details
Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- RNA polymerase sigma-70
- DNA-directed RNA polymerase, insert domain superfamily
- DNA-directed RNA polymerase, RpoA/D/Rpb3-type
- RNA polymerase, RBP11-like subunit
- DNA-directed RNA polymerase, alpha subunit
- Winged helix-like DNA-binding domain superfamily
- RNA polymerase sigma factor, region 3/4-like
- RNA polymerase, subunit omega/Rpo6/RPB6
36 more domains
Structure analysis Details
Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-118267 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
2 distinct DNA molecules
Macromolecules (7 distinct):
DNA-directed RNA polymerase subunit alpha
Molecule details ›
Chains: A, B, G, H
Length: 314 amino acids
Theoretical weight: 34.83 KDa
Source organism: Thermus aquaticus
UniProt:
Sequence domains:
Length: 314 amino acids
Theoretical weight: 34.83 KDa
Source organism: Thermus aquaticus
UniProt:
- Canonical:
Q9KWU8 (Residues: 1-314; Coverage: 100%)
Sequence domains:
DNA-directed RNA polymerase subunit beta
Molecule details ›
Chains: C, I
Length: 1119 amino acids
Theoretical weight: 124.93 KDa
Source organism: Thermus aquaticus
UniProt:
Sequence domains:
Length: 1119 amino acids
Theoretical weight: 124.93 KDa
Source organism: Thermus aquaticus
UniProt:
- Canonical: Q9KWU7 (Residues: 1-1119; Coverage: 100%)
Sequence domains:
DNA-directed RNA polymerase subunit beta'
Molecule details ›
Chains: D, J
Length: 1524 amino acids
Theoretical weight: 171.19 KDa
Source organism: Thermus aquaticus
UniProt:
Sequence domains:
Length: 1524 amino acids
Theoretical weight: 171.19 KDa
Source organism: Thermus aquaticus
UniProt:
- Canonical: Q9KWU6 (Residues: 1-1524; Coverage: 100%)
Sequence domains:
DNA-directed RNA polymerase subunit omega
Molecule details ›
Chains: E, K
Length: 99 amino acids
Theoretical weight: 11.64 KDa
Source organism: Thermus aquaticus
UniProt:
Sequence domains: RNA polymerase Rpb6
Length: 99 amino acids
Theoretical weight: 11.64 KDa
Source organism: Thermus aquaticus
UniProt:
- Canonical: Q9EVV4 (Residues: 1-99; Coverage: 100%)
Sequence domains: RNA polymerase Rpb6
RNA polymerase sigma factor SigA
Molecule details ›
Chains: F, L
Length: 347 amino acids
Theoretical weight: 39.86 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 347 amino acids
Theoretical weight: 39.86 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: Q9EZJ8 (Residues: 92-438; Coverage: 79%)
Sequence domains:
