› 4xrh

X-ray diffraction
3Å resolution

Human DNA polymerase lambda- MgdTTP binary and complex with 6 paired DNA

Released:
DOI: 10.2210/pdb4xrh/pdb
PDB entry 4xrh coloured by chain, front view.
PDB entry 4xrh coloured by chain, side view.
PDB entry 4xrh coloured by chain, top view.
Source organisms:
Primary publication:
Structural Mechanism for the Fidelity Modulation of DNA Polymerase λ.
Liu MS, Tsai HY, Liu XX, Ho MC, Wu WJ, Tsai MD
J Am Chem Soc 138 2389-98 (2016)
PMID: 26836966

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-118285 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase lambda Chains: A, B
Molecule details ›
Chains: A, B
Length: 335 amino acids
Theoretical weight: 37.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UGP5 (Residues: 242-575; Coverage: 58%)
Gene name: POLL
Sequence domains:
Structure domains:
DNA (5'-D(*CP*AP*GP*TP*AP*C)-3') Chain: P
Molecule details ›
Chain: P
Length: 6 nucleotides
Theoretical weight: 1.79 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*GP*TP*AP*CP*TP*G)-3') Chain: T
Molecule details ›
Chain: T
Length: 6 nucleotides
Theoretical weight: 1.82 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand TTP 1 x TTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P6522
Unit cell:
a: 84.083Å b: 84.083Å c: 397.76Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.218 0.251
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Nonhomologous DNA end-joining for repair of DNA double-strand breaks.
Pannunzio et al. (2018)
1 more