› 4xrm

X-ray diffraction
1.6Å resolution

homodimer of TALE type homeobox transcription factor MEIS1 complexes with specific DNA

Released:
DOI: 10.2210/pdb4xrm/pdb
PDB entry 4xrm coloured by chain, front view.
PDB entry 4xrm coloured by chain, side view.
PDB entry 4xrm coloured by chain, top view.
Source organisms:
Primary publication:
DNA-dependent formation of transcription factor pairs alters their binding specificity.
Jolma A, Yin Y, Nitta KR, Dave K, Popov A, Taipale M, Enge M, Kivioja T, Morgunova E, Taipale J
Nature 527 384-8 (2015)
PMID: 26550823

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118286 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Homeobox protein Meis2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 64 amino acids
Theoretical weight: 7.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14770 (Residues: 281-342; Coverage: 13%)
Gene names: MEIS2, MRG1
Sequence domains: Homeobox KN domain
Structure domains: Homeodomain-like
DNA (5'-D(P*AP*GP*CP*TP*GP*AP*CP*AP*GP*CP*TP*GP*TP*CP*AP*AP*G)-3') Chain: M
Molecule details ›
Chain: M
Length: 17 nucleotides
Theoretical weight: 5.24 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*TP*CP*TP*TP*GP*AP*CP*AP*GP*CP*TP*GP*TP*CP*AP*GP*C)-3') Chain: L
Molecule details ›
Chain: L
Length: 17 nucleotides
Theoretical weight: 5.18 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand TRS 2 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: I222
Unit cell:
a: 70.62Å b: 79.28Å c: 98.97Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.177 0.211
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

53 review citations

Ever-Changing Landscapes: Transcriptional Enhancers in Development and Evolution.
Long et al. (2016)
52 more

6 mentions without citation

Detecting and interpreting DNA methylation marks.
Ren et al. (2018)
5 more