› 4xsj

X-ray diffraction
1.8Å resolution

Crystal structure of the N-terminal domain of the human mitochondrial calcium uniporter fused with T4 lysozyme

Released:
DOI: 10.2210/pdb4xsj/pdb
PDB entry 4xsj coloured by chain, front view.
PDB entry 4xsj coloured by chain, side view.
PDB entry 4xsj coloured by chain, top view.
Source organisms:
Primary publication:
Structure and function of the N-terminal domain of the human mitochondrial calcium uniporter.
Lee Y, Min CK, Kim TG, Song HK, Lim Y, Kim D, Shin K, Kang M, Kang JY, Youn HS, Lee JG, An JY, Park KR, Lim JJ, Kim JH, Kim JH, Park ZY, Kim YS, Wang J, Kim DH, Eom SH
EMBO Rep 16 1318-33 (2015)
PMID: 26341627

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112947 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium uniporter protein, mitochondrial; Endolysin Chain: A
Molecule details ›
Chain: A
Length: 262 amino acids
Theoretical weight: 29.79 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: D9IEF7 (Residues: 1-161; Coverage: 98%)
  • Canonical: Q8NE86 (Residues: 75-165; Coverage: 26%)
Gene names: C10orf42, CCDC109A, MCU, T4Tp126, e
Sequence domains: Phage lysozyme

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P65
Unit cell:
a: 98.085Å b: 98.085Å c: 62.409Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.13 0.127 0.19
Expression system: Escherichia coli

Quick links

Citations

24 review citations

The machineries, regulation and cellular functions of mitochondrial calcium.
Giorgi et al. (2018)
23 more

3 mentions without citation

Mitochondrial Ca2+ transport in the endothelium: regulation by ions, redox signalling and mechanical forces.
Alevriadou et al. (2017)
2 more