› 4xzb

X-ray diffraction
1.62Å resolution

endo-glucanase GsCelA P1

Released:
DOI: 10.2210/pdb4xzb/pdb
PDB entry 4xzb coloured by chain, front view.
PDB entry 4xzb coloured by chain, side view.
PDB entry 4xzb coloured by chain, top view.
Source organism: Geobacillus sp. 70PC53
Entry authors: Lee CC, Chang CJ, Ho THD, Chao YC, Wang AHJ

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111497 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cellulase Chain: A
Molecule details ›
Chain: A
Length: 314 amino acids
Theoretical weight: 35.8 KDa
Source organism: Geobacillus sp. 70PC53
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C5H6X3 (Residues: 25-330; Coverage: 78%)
Gene name: celA
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 40.957Å b: 71.673Å c: 92.248Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.159 0.178
Expression system: Escherichia coli BL21(DE3)

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