› 4y18

X-ray diffraction
3.5Å resolution

Structure of BRCA1 BRCT domains in complex with Abraxas double phosphorylated peptide

Released:
DOI: 10.2210/pdb4y18/pdb
PDB entry 4y18 coloured by chain, front view.
PDB entry 4y18 coloured by chain, side view.
PDB entry 4y18 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of BRCA1-BRCT/Abraxas Complex Reveals Phosphorylation-Dependent BRCT Dimerization at DNA Damage Sites.
Wu Q, Paul A, Su D, Mehmood S, Foo TK, Ochi T, Bunting EL, Xia B, Robinson CV, Wang B, Blundell TL
Mol Cell 61 434-448 (2016)
PMID: 26778126

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-153638 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Breast cancer type 1 susceptibility protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 224 amino acids
Theoretical weight: 25.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P38398 (Residues: 1646-1859; Coverage: 12%)
Gene names: BRCA1, RNF53
Sequence domains: BRCA1 C Terminus (BRCT) domain
Structure domains: BRCT domain
BRCA1-A complex subunit Abraxas 1 Chains: I, J, K, L, M, N, O, P
Molecule details ›
Chains: I, J, K, L, M, N, O, P
Length: 11 amino acids
Theoretical weight: 1.41 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q6UWZ7 (Residues: 399-409; Coverage: 3%)
Gene names: ABRA1, ABRAXAS1, CCDC98, FAM175A, UNQ496/PRO1013

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 16 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 86.819Å b: 183.726Å c: 190.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.233 0.298
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

DNA damage response signaling pathways and targets for radiotherapy sensitization in cancer.
Huang et al. (2020)
6 more

3 mentions without citation

Fanconi anemia pathway as a prospective target for cancer intervention.
Liu et al. (2020)
2 more