› 4y4l

X-ray diffraction
2Å resolution

Crystal structure of yeast Thi4-C205S

Released:
DOI: 10.2210/pdb4y4l/pdb
PDB entry 4y4l coloured by chain, front view.
PDB entry 4y4l coloured by chain, side view.
PDB entry 4y4l coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural Basis for Iron-Mediated Sulfur Transfer in Archael and Yeast Thiazole Synthases.
Zhang X, Eser BE, Chanani PK, Begley TP, Ealick SE
Biochemistry 55 1826-38 (2016)
PMID: 26919468

Function and Biology Details

Reaction catalysed: 
NAD(+) + glycine + [ADP-thiazole synthase]-L-cysteine = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152255 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiamine thiazole synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 346 amino acids
Theoretical weight: 37.19 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32318 (Residues: 1-326; Coverage: 100%)
Gene names: ESP35, G6620, MOL1, THI4, YGR144W
Sequence domains: Thi4 family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 48N 4 x 48N
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P4
Unit cell:
a: 127.393Å b: 127.393Å c: 72.579Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.192
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Vitamin Biosynthesis as an Antifungal Target.
Meir et al. (2018)
1 more

1 mention without citation

Structural Basis for Iron-Mediated Sulfur Transfer in Archael and Yeast Thiazole Synthases.
Zhang et al. (2016)