› 4y7o

X-ray diffraction
2.24Å resolution

T6SS protein TssM C-terminal domain (869-1107) from EAEC

Released:
DOI: 10.2210/pdb4y7o/pdb
PDB entry 4y7o coloured by chain, front view.
PDB entry 4y7o coloured by chain, side view.
PDB entry 4y7o coloured by chain, top view.
Source organisms:
Primary publication:
Biogenesis and structure of a type VI secretion membrane core complex.
Durand E, Nguyen VS, Zoued A, Logger L, Péhau-Arnaudet G, Aschtgen MS, Spinelli S, Desmyter A, Bardiaux B, Dujeancourt A, Roussel A, Cambillau C, Cascales E, Fronzes R
Nature 523 555-60 (2015)
PMID: 26200339

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102458 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Type VI secretion protein IcmF Chains: A, B
Molecule details ›
Chains: A, B
Length: 240 amino acids
Theoretical weight: 26.51 KDa
Source organism: Escherichia coli 1-176-05_S3_C1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0M3KL17 (Residues: 1-240; Coverage: 100%)
Gene name: AB98_0769
Sequence domains:
Type VI secretion system protein VasD Chains: C, D
Molecule details ›
Chains: C, D
Length: 155 amino acids
Theoretical weight: 16.98 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
Structure domains: Type VI secretion system, lipoprotein SciN

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P41212
Unit cell:
a: 85.47Å b: 85.47Å c: 256.37Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.204 0.224
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

32 review citations

Aim, Load, Fire: The Type VI Secretion System, a Bacterial Nanoweapon.
Cianfanelli et al. (2016)
31 more

3 mentions without citation

Domestication of a housekeeping transglycosylase for assembly of a Type VI secretion system.
Santin et al. (2017)
2 more