› 4yhg

X-ray diffraction
2.4Å resolution

NATIVE BACTEROIDETES-AFFILIATED GH5 CELLULASE LINKED WITH A POLYSACCHARIDE UTILIZATION LOCUS

Released:
DOI: 10.2210/pdb4yhg/pdb
PDB entry 4yhg coloured by chain, front view.
PDB entry 4yhg coloured by chain, side view.
PDB entry 4yhg coloured by chain, top view.
Source organism: Bacteroidetes bacterium AC2a
Primary publication:
Structural Features of a Bacteroidetes-Affiliated Cellulase Linked with a Polysaccharide Utilization Locus.
Naas AE, MacKenzie AK, Dalhus B, Eijsink VGH, Pope PB
Sci Rep 5 11666 (2015)
PMID: 26133573

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100485 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Glycoside hydrolase family 5 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 389 amino acids
Theoretical weight: 43.62 KDa
Source organism: Bacteroidetes bacterium AC2a
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A076MPD7 (Residues: 26-414; Coverage: 99%)
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 84.326Å b: 99.553Å c: 102.462Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.228
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Polysaccharide Utilization Loci: Fueling Microbial Communities.
Grondin et al. (2017)
1 more

2 mentions without citation

Metadata Analysis Approaches for Understanding and Improving the Functional Involvement of Rumen Microbial Consortium in Digestion and Metabolism of Plant Biomass.
Kameshwar et al. (2019)
1 more