› 4yi8

X-ray diffraction
1.2Å resolution

Crystal structure of non-myristoylated E153A recoverin at 1.2 A resolution with calcium ions bound to EF-hands 2 and 3

Released:
DOI: 10.2210/pdb4yi8/pdb
PDB entry 4yi8 coloured by chain, front view.
PDB entry 4yi8 coloured by chain, side view.
PDB entry 4yi8 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Crystal Structure of Recoverin with Calcium Ions Bound to Both Functional EF Hands.
Kumar RP, Ranaghan MJ, Ganjei AY, Oprian DD
Biochemistry 54 7222-8 (2015)
PMID: 26584024

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149260 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Recoverin Chain: A
Molecule details ›
Chain: A
Length: 201 amino acids
Theoretical weight: 23.18 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P21457 (Residues: 2-202; Coverage: 100%)
Gene names: RCV1, RCVRN
Sequence domains: EF hand
Structure domains: EF-hand

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: I4
Unit cell:
a: 84.16Å b: 84.16Å c: 59.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.122 0.121 0.14
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Zebrafish Recoverin Isoforms Display Differences in Calcium Switch Mechanisms.
Elbers et al. (2018)
1 more

5 mentions without citation

Crystal Structure of Recoverin with Calcium Ions Bound to Both Functional EF Hands.
Kumar et al. (2015)
4 more