› 4zlp

X-ray diffraction
2.48Å resolution

Crystal Structure of Notch3 Negative Regulatory Region

Released:
DOI: 10.2210/pdb4zlp/pdb
PDB entry 4zlp coloured by chain, front view.
PDB entry 4zlp coloured by chain, side view.
PDB entry 4zlp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Insights into Autoregulation of Notch3 from Structural and Functional Studies of Its Negative Regulatory Region.
Xu X, Choi SH, Hu T, Tiyanont K, Habets R, Groot AJ, Vooijs M, Aster JC, Chopra R, Fryer C, Blacklow SC
Structure 23 1227-35 (2015)
PMID: 26051713

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193959 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Notch 3 extracellular truncation Chains: A, B
Molecule details ›
Chains: A, B
Length: 271 amino acids
Theoretical weight: 30.1 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9UM47 (Residues: 1378-1640; Coverage: 12%)
Gene name: NOTCH3
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
4 bound ligands:
Ligand CA 6 x CA
Ligand GOL 3 x GOL
Ligand ACT 2 x ACT
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 122.482Å b: 64.31Å c: 83.089Å
α: 90° β: 105.23° γ: 90°
R-values:
R R work R free
0.18 0.178 0.204
Expression system: Homo sapiens

Quick links

Citations

14 review citations

Notch Signaling in Development, Tissue Homeostasis, and Disease.
Siebel et al. (2017)
13 more

5 mentions without citation

Insights into Autoregulation of Notch3 from Structural and Functional Studies of Its Negative Regulatory Region.
Xu et al. (2015)
4 more