› 4zrj

X-ray diffraction
2.3Å resolution

Structure of Merlin-FERM and CTD

Released:
DOI: 10.2210/pdb4zrj/pdb
PDB entry 4zrj coloured by chain, front view.
PDB entry 4zrj coloured by chain, side view.
PDB entry 4zrj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Angiomotin binding-induced activation of Merlin/NF2 in the Hippo pathway.
Li Y, Zhou H, Li F, Chan SW, Lin Z, Wei Z, Yang Z, Guo F, Lim CJ, Xing W, Shen Y, Hong W, Long J, Zhang M
Cell Res 25 801-17 (2015)
PMID: 26045165

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152865 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Merlin Chain: A
Molecule details ›
Chain: A
Length: 320 amino acids
Theoretical weight: 37.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35240 (Residues: 1-320; Coverage: 54%)
Gene names: NF2, SCH
Sequence domains:
Structure domains:
Merlin Chain: B
Molecule details ›
Chain: B
Length: 90 amino acids
Theoretical weight: 10.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35240 (Residues: 506-595; Coverage: 15%)
Gene names: NF2, SCH
Sequence domains: Ezrin/radixin/moesin family C terminal

Ligands and Environments

1 bound ligand:
Ligand GOL 8 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 67.895Å b: 68.202Å c: 82.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.186 0.248
Expression system: Escherichia coli

Quick links

Citations

41 review citations

Mechanisms of Hippo pathway regulation.
Meng et al. (2016)
40 more

10 mentions without citation

Two Sides of the Coin: Ezrin/Radixin/Moesin and Merlin Control Membrane Structure and Contact Inhibition.
Michie et al. (2019)
9 more