› 4zxb

X-ray diffraction
3.3Å resolution

Structure of the human insulin receptor ectodomain, IRDeltabeta construct, in complex with four Fab molecules

Released:
DOI: 10.2210/pdb4zxb/pdb
PDB entry 4zxb coloured by chain, front view.
PDB entry 4zxb coloured by chain, side view.
PDB entry 4zxb coloured by chain, top view.
Source organisms:
Primary publication:
Higher-Resolution Structure of the Human Insulin Receptor Ectodomain: Multi-Modal Inclusion of the Insert Domain.
Croll TI, Smith BJ, Margetts MB, Whittaker J, Weiss MA, Ward CW, Lawrence MC
Structure 24 469-76 (2016)
PMID: 26853939

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-106915 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (9 distinct):
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 23.41 KDa
Source organism: Mus musculus
Expression system: Mus musculus
UniProt:
  • Canonical: U5LP42 (Residues: 20-118, 119-240; Coverage: 49%)
Sequence domains:
Structure domains: Immunoglobulins
Immunoglobulin kappa constant Chain: B
Molecule details ›
Chain: B
Length: 219 amino acids
Theoretical weight: 24.39 KDa
Source organism: Mus musculus
Expression system: Mus musculus
UniProt:
  • Canonical: P01837 (Residues: 1-107; Coverage: 100%)
Gene name: Igkc
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Ig gamma-2A chain C region, membrane-bound form Chain: C
Molecule details ›
Chain: C
Length: 220 amino acids
Theoretical weight: 23.62 KDa
Source organism: Mus musculus
Expression system: Mus musculus
UniProt:
  • Canonical: P01865 (Residues: 1-106; Coverage: 27%)
Gene name: Igh-1a
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chain: D
Molecule details ›
Chain: D
Length: 214 amino acids
Theoretical weight: 23.51 KDa
Source organism: Mus musculus
Expression system: Mus musculus
UniProt:
  • Canonical: A2P1G9 (Residues: 1-219; Coverage: 98%)
Gene names: Igk-C, Igkc
Sequence domains:
Structure domains: Immunoglobulins
Insulin receptor subunit alpha Chain: E
Molecule details ›
Chain: E
Length: 894 amino acids
Theoretical weight: 102.28 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P06213 (Residues: 28-956; Coverage: 66%)
Gene name: INSR
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
1 bound ligand:
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: C2221
Unit cell:
a: 121.87Å b: 321.25Å c: 199.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.237
Expression systems:
  • Mus musculus
  • Cricetulus griseus

Quick links

Citations

3 review citations

The Insulin Receptor: An Important Target for the Development of Novel Medicines and Pesticides.
Zhang et al. (2022)
2 more

37 mentions without citation

Insulin Receptor Isoforms in Physiology and Disease: An Updated View.
Belfiore et al. (2017)
36 more