› 4zxf

X-ray diffraction
2.5Å resolution

Crystal Structure of a Soluble Variant of Monoglyceride Lipase from Saccharomyces Cerevisiae in Complex with a Substrate Analog

Released:
DOI: 10.2210/pdb4zxf/pdb
PDB entry 4zxf coloured by chain, front view.
PDB entry 4zxf coloured by chain, side view.
PDB entry 4zxf coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Crystal structure of the Saccharomyces cerevisiae monoglyceride lipase Yju3p.
Aschauer P, Rengachari S, Lichtenegger J, Schittmayer M, Das KM, Mayer N, Breinbauer R, Birner-Gruenberger R, Gruber CC, Zimmermann R, Gruber K, Oberer M
Biochim Biophys Acta 1861 462-70 (2016)
PMID: 26869448

Function and Biology Details

Reaction catalysed: 
Hydrolyzes glycerol monoesters of long-chain fatty acids
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151203 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monoglyceride lipase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 340 amino acids
Theoretical weight: 38.91 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28321 (Residues: 1-313; Coverage: 100%)
Gene names: YJU3, YKL094W, YKL441
Sequence domains: Serine aminopeptidase, S33
Structure domains: alpha/beta hydrolase

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand 4S7 1 x 4S7
Ligand NO3 2 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 76.76Å b: 107.13Å c: 165.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.201 0.257
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 citation in other articles

Hypoxia-inducible lipid droplet-associated protein inhibits adipose triglyceride lipase.
Padmanabha Das et al. (2018)
8 more

3 mentions without citation

A Thermostable Monoacylglycerol Lipase from Marine Geobacillus sp. 12AMOR1: Biochemical Characterization and Mutagenesis Study.
Tang et al. (2019)
2 more