› 4zz7

X-ray diffraction
2.9Å resolution

Crystal structure of methylmalonate-semialdehyde dehydrogenase (DddC) from Oceanimonas doudoroffii

Released:
DOI: 10.2210/pdb4zz7/pdb
PDB entry 4zz7 coloured by chain, front view.
PDB entry 4zz7 coloured by chain, side view.
PDB entry 4zz7 coloured by chain, top view.
Source organism: Oceanimonas doudoroffii
Primary publication:
Crystal structure and modeling of the tetrahedral intermediate state of methylmalonate-semialdehyde dehydrogenase (MMSDH) from Oceanimonas doudoroffii.
Do H, Lee CW, Lee SG, Kang H, Park CM, Kim HJ, Park H, Park H, Lee JH
J Microbiol 54 114-21 (2016)
PMID: 26832667

Function and Biology Details

Reaction catalysed: 
2-methyl-3-oxopropanoate + CoA + H(2)O + NAD(+) = propanoyl-CoA + HCO(3)(-) + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124478 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
methylmalonate-semialdehyde dehydrogenase (CoA acylating) Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 501 amino acids
Theoretical weight: 54.37 KDa
Source organism: Oceanimonas doudoroffii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G5CZI2 (Residues: 1-498; Coverage: 100%)
Gene names: B6S08_06410, dddC, mmsA
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 6 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21212
Unit cell:
a: 156.7Å b: 160.3Å c: 238.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.185 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

NAD+ promotes assembly of the active tetramer of aldehyde dehydrogenase 7A1.
Korasick et al. (2018)
1 more

2 mentions without citation

Expression and Interaction Analysis among Saffron ALDHs and Crocetin Dialdehyde.
Gómez-Gómez et al. (2018)
1 more