› 5a0f

X-ray diffraction
2Å resolution

Crystal structure of Yersinia Afp18-modified RhoA

Released:
DOI: 10.2210/pdb5a0f/pdb
PDB entry 5a0f coloured by chain, front view.
PDB entry 5a0f coloured by chain, side view.
PDB entry 5a0f coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Tyrosine glycosylation of Rho by Yersinia toxin impairs blastomere cell behaviour in zebrafish embryos.
Jank T, Eckerle S, Steinemann M, Trillhaase C, Schimpl M, Wiese S, van Aalten DM, Driever W, Aktories K
Nat Commun 6 7807 (2015)
PMID: 26190758

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158275 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transforming protein RhoA Chain: A
Molecule details ›
Chain: A
Length: 181 amino acids
Theoretical weight: 20.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61586 (Residues: 1-181; Coverage: 94%)
Gene names: ARH12, ARHA, RHO12, RHOA
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand GDP 1 x GDP
Ligand SO4 1 x SO4
Ligand NDG 1 x NDG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P43212
Unit cell:
a: 91.392Å b: 91.392Å c: 56.628Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.205 0.213
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

The pyrin inflammasome: from sensing RhoA GTPases-inhibiting toxins to triggering autoinflammatory syndromes.
Jamilloux et al. (2018)
8 more

2 mentions without citation

Tyrosine glycosylation of Rho by Yersinia toxin impairs blastomere cell behaviour in zebrafish embryos.
Jank et al. (2015)
1 more