› 5a2h

X-ray diffraction
2.27Å resolution

Crystal Structure of Arabidopsis thaliana Calmodulin-7

Released:
DOI: 10.2210/pdb5a2h/pdb
PDB entry 5a2h coloured by chain, front view.
PDB entry 5a2h coloured by chain, side view.
PDB entry 5a2h coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Crystal structure of Arabidopsis thaliana calmodulin7 and insight into its mode of DNA binding.
Kumar S, Mazumder M, Gupta N, Chattopadhyay S, Gourinath S
FEBS Lett 590 3029-39 (2016)
PMID: 27500568

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157780 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calmodulin-7 Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.86 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P59220 (Residues: 1-149; Coverage: 100%)
Gene names: At3g43810, CAM7, T28A8.100
Sequence domains: EF-hand domain pair

Ligands and Environments

3 bound ligands:
Ligand CA 4 x CA
Ligand MPD 3 x MPD
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 24.455Å b: 67.901Å c: 111.29Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

In Vitro Analytical Approaches to Study Plant Ligand-Receptor Interactions.
Sandoval et al. (2020)
3 more

2 mentions without citation

Towards Understanding Plant Calcium Signaling through Calmodulin-Like Proteins: A Biochemical and Structural Perspective.
La Verde et al. (2018)
1 more