› 5acl

X-ray diffraction
1.49Å resolution

Mcg immunoglobulin variable domain with sulfasalazine

Released:
DOI: 10.2210/pdb5acl/pdb
PDB entry 5acl coloured by chain, front view.
PDB entry 5acl coloured by chain, side view.
PDB entry 5acl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Inhibition by small-molecule ligands of formation of amyloid fibrils of an immunoglobulin light chain variable domain.
Brumshtein B, Esswein SR, Salwinski L, Phillips ML, Ly AT, Cascio D, Sawaya MR, Eisenberg DS
Elife 4 e10935 (2015)
PMID: 26576950

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134489 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin lambda variable 2-8 Chain: A
Molecule details ›
Chain: A
Length: 111 amino acids
Theoretical weight: 11.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01709 (Residues: 20-118; Coverage: 100%)
Gene name: IGLV2-8
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand SAS 1 x SAS
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21212
Unit cell:
a: 31.86Å b: 74.61Å c: 39.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.158 0.187
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

AL amyloidosis: from molecular mechanisms to targeted therapies.
Merlini G. (2017)
9 more

4 mentions without citation

Inhibition by small-molecule ligands of formation of amyloid fibrils of an immunoglobulin light chain variable domain.
Brumshtein et al. (2015)
3 more