› 5afq

X-ray diffraction
7Å resolution

Crystal structure of RPC62 - RPC32 beta

Released:
DOI: 10.2210/pdb5afq/pdb
PDB entry 5afq coloured by chain, front view.
PDB entry 5afq coloured by chain, side view.
PDB entry 5afq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of human RPC32β-RPC62 complex.
Boissier F, Dumay-Odelot H, Teichmann M, Fribourg S
J Struct Biol 192 313-319 (2015)
PMID: 26394183

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165434 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA-directed RNA polymerase III subunit RPC3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 534 amino acids
Theoretical weight: 60.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BUI4 (Residues: 1-534; Coverage: 100%)
Gene name: POLR3C
Sequence domains:
RPC32 BETA (RPC7L) Chains: D, E
Molecule details ›
Chains: D, E
Length: 218 amino acids
Theoretical weight: 18.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: I422
Unit cell:
a: 218.27Å b: 218.27Å c: 182.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.271 0.268 0.298
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

RNA Polymerase III Advances: Structural and tRNA Functional Views.
Arimbasseri et al. (2016)
2 more

2 mentions without citation

Cryo-EM structures of human RNA polymerase III in its unbound and transcribing states.
Girbig et al. (2021)
1 more