› 5ahr

X-ray diffraction
2.19Å resolution

Crystal structure of human DNA cross-link repair 1A, crystal form B

Released:
DOI: 10.2210/pdb5ahr/pdb
PDB entry 5ahr coloured by chain, front view.
PDB entry 5ahr coloured by chain, side view.
PDB entry 5ahr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structures of the SNM1A and SNM1B/Apollo nuclease domains reveal a potential basis for their distinct DNA processing activities.
Allerston CK, Lee SY, Newman JA, Schofield CJ, McHugh PJ, Gileadi O
Nucleic Acids Res 43 11047-60 (2015)
PMID: 26582912

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180186 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA cross-link repair 1A protein Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 38.88 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q6PJP8 (Residues: 700-1040; Coverage: 33%)
Gene names: DCLRE1A, KIAA0086, SNM1, SNM1A
Sequence domains: DNA repair metallo-beta-lactamase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P41212
Unit cell:
a: 113.199Å b: 113.199Å c: 125.736Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.179 0.201
Expression system: Spodoptera frugiperda

Quick links

Citations

4 review citations

Involvement of translesion synthesis DNA polymerases in DNA interstrand crosslink repair.
Roy et al. (2016)
3 more

5 mentions without citation

The SNM1A DNA repair nuclease.
Baddock et al. (2020)
4 more