Function and Biology Details
Reaction catalysed:
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Sequence domains:
Structure analysis Details
Assembly composition:
monomeric (preferred)
Assembly name:
E3 ubiquitin-protein ligase NEDD4 (preferred)
PDBe Complex ID:
PDB-CPX-155546 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase NEDD4
Molecule details ›
Chain: A
Length: 43 amino acids
Theoretical weight: 4.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: WW domain
Structure domains: Ubiquitin Ligase Nedd4; Chain: W;
Length: 43 amino acids
Theoretical weight: 4.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P46934 (Residues: 838-877; Coverage: 3%)
Sequence domains: WW domain
Structure domains: Ubiquitin Ligase Nedd4; Chain: W;
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
Chemical shift assignment:
87%
Refinement method:
SEMI-AUTOMATED NOE ASSIGNMENT, SIMULATED ANNEALING
Expression system: Escherichia coli BL21(DE3)
