› 5ahv

Electron Microscopy
13.6Å resolution

Cryo-EM structure of helical ANTH and ENTH tubules on PI(4,5)P2-containing membranes

Released:
DOI: 10.2210/pdb5ahv/pdb
PDB entry 5ahv coloured by chain, front view.
PDB entry 5ahv coloured by chain, side view.
PDB entry 5ahv coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
An organized co-assembly of clathrin adaptors is essential for endocytosis.
Skruzny M, Desfosses A, Prinz S, Dodonova SO, Gieras A, Uetrecht C, Jakobi AJ, Abella M, Hagen WJ, Schulz J, Meijers R, Rybin V, Briggs JA, Sachse C, Kaksonen M
Dev Cell 33 150-62 (2015)
PMID: 25898165
Related structures: EMD-2896

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 156-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152598 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Epsin-1 Chain: E
Molecule details ›
Chain: E
Length: 154 amino acids
Theoretical weight: 18.01 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12518 (Residues: 1-154; Coverage: 34%)
Gene names: ENT1, YDL161W
Sequence domains: ENTH domain
Protein SLA2 Chain: F
Molecule details ›
Chain: F
Length: 268 amino acids
Theoretical weight: 30.7 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P33338 (Residues: 5-272; Coverage: 28%)
Gene names: END4, MOP2, N1102, SLA2, UFG1, YNL243W
Sequence domains: ANTH domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 13.6Å
Relevant EMDB volumes: EMD-2896
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Mechanisms of clathrin-mediated endocytosis.
Kaksonen et al. (2018)
10 more