5ajj

X-ray diffraction
1.75Å resolution

Crystal structure of variola virus virulence factor F1L in complex with human Bid BH3 domain

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-157372 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Apoptosis regulator OPG045 Chain: A
Molecule details ›
Chain: A
Length: 168 amino acids
Theoretical weight: 19.65 KDa
Source organism: Variola virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q85365 (Residues: 39-201; Coverage: 69%)
Gene names: C5L, OPG045
Sequence domains: Apoptosis regulator M11L like
BH3-interacting domain death agonist p11 Chain: B
Molecule details ›
Chain: B
Length: 34 amino acids
Theoretical weight: 3.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P55957 (Residues: 79-112; Coverage: 17%)
Gene name: BID

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: F222
Unit cell:
a: 52.094Å b: 112.318Å c: 117.178Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.167 0.208
Expression system: Escherichia coli BL21(DE3)