› 5ajk

X-ray diffraction
2.55Å resolution

Crystal structure of variola virus virulence factor F1L in complex with human Bak BH3 domain

Released:
DOI: 10.2210/pdb5ajk/pdb
PDB entry 5ajk coloured by chain, front view.
PDB entry 5ajk coloured by chain, side view.
PDB entry 5ajk coloured by chain, top view.
Source organisms:
Primary publication:
Variola virus F1L is a Bcl-2-like protein that unlike its vaccinia virus counterpart inhibits apoptosis independent of Bim.
Marshall B, Puthalakath H, Caria S, Chugh S, Doerflinger M, Colman PM, Kvansakul M
Cell Death Dis 6 e1680 (2015)
PMID: 25766319

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172525 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Apoptosis regulator OPG045 Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 168 amino acids
Theoretical weight: 19.65 KDa
Source organism: Variola virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q85365 (Residues: 39-201; Coverage: 69%)
Gene names: C5L, OPG045
Sequence domains: Apoptosis regulator M11L like
Bcl-2 homologous antagonist/killer Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 26 amino acids
Theoretical weight: 2.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q16611 (Residues: 67-92; Coverage: 12%)
Gene names: BAK, BAK1, BCL2L7, CDN1

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand ACT 3 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Unit cell:
a: 124.774Å b: 68.762Å c: 171.598Å
α: 90° β: 109.43° γ: 90°
R-values:
R R work R free
0.199 0.197 0.24
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Inflammasomes and its importance in viral infections.
Shrivastava et al. (2016)
7 more

1 mention without citation

Variola virus F1L is a Bcl-2-like protein that unlike its vaccinia virus counterpart inhibits apoptosis independent of Bim.
Marshall et al. (2015)