› 5anr

X-ray diffraction
2.1Å resolution

Structure of a human 4E-T - DDX6 - CNOT1 complex

Released:
DOI: 10.2210/pdb5anr/pdb
PDB entry 5anr coloured by chain, front view.
PDB entry 5anr coloured by chain, side view.
PDB entry 5anr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of a Human 4E-T/DDX6/CNOT1 Complex Reveals the Different Interplay of DDX6-Binding Proteins with the CCR4-NOT Complex.
Ozgur S, Basquin J, Kamenska A, Filipowicz W, Standart N, Conti E
Cell Rep 13 703-711 (2015)
PMID: 26489469

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-107788 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
CCR4-NOT transcription complex subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 29.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A5YKK6 (Residues: 1063-1314; Coverage: 11%)
Gene names: AD-005, CDC39, CNOT1, KIAA1007, NOT1
Sequence domains: CCR4-NOT transcription complex subunit 1 CAF1-binding domain
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
Probable ATP-dependent RNA helicase DDX6 Chain: B
Molecule details ›
Chain: B
Length: 378 amino acids
Theoretical weight: 43.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P26196 (Residues: 95-469; Coverage: 78%)
Gene names: DDX6, HLR2, RCK
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Eukaryotic translation initiation factor 4E transporter Chain: C
Molecule details ›
Chain: C
Length: 44 amino acids
Theoretical weight: 5.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9NRA8 (Residues: 199-239; Coverage: 4%)
Gene name: EIF4ENIF1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3221
Unit cell:
a: 93.129Å b: 93.129Å c: 175.329Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.193 0.232
Expression system: Escherichia coli BL21

Quick links

Citations

17 review citations

Metazoan MicroRNAs.
Bartel DP. (2018)
16 more

6 mentions without citation

Host DDX Helicases as Possible SARS-CoV-2 Proviral Factors: A Structural Overview of Their Hijacking Through Multiple Viral Proteins.
Squeglia et al. (2020)
5 more