› 5ao4

X-ray diffraction
3.7Å resolution

Crystal structure of in vitro phosphorylated human SAMHD1 (amino acid residues 115-626) bound to GTP

Released:
DOI: 10.2210/pdb5ao4/pdb
PDB entry 5ao4 coloured by chain, front view.
PDB entry 5ao4 coloured by chain, side view.
PDB entry 5ao4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Phospho-dependent Regulation of SAMHD1 Oligomerisation Couples Catalysis and Restriction.
Arnold LH, Groom HC, Kunzelmann S, Schwefel D, Caswell SJ, Ordonez P, Mann MC, Rueschenbaum S, Goldstone DC, Pennell S, Howell SA, Stoye JP, Webb M, Taylor IA, Bishop KN
PLoS Pathog 11 e1005194 (2015)
PMID: 26431200

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195223 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 538 amino acids
Theoretical weight: 62.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y3Z3 (Residues: 115-626; Coverage: 82%)
Gene names: MOP5, SAMHD1
Sequence domains: HD domain
Structure domains: Hypothetical protein af1432

Ligands and Environments

2 bound ligands:
Ligand FE 4 x FE
Ligand GTP 4 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2
Unit cell:
a: 197.545Å b: 80.817Å c: 147.665Å
α: 90° β: 114.93° γ: 90°
R-values:
R R work R free
0.247 0.244 0.308
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

From APOBEC to ZAP: Diverse mechanisms used by cellular restriction factors to inhibit virus infections.
Chemudupati et al. (2019)
11 more

3 mentions without citation

SAMHD1: Recurring roles in cell cycle, viral restriction, cancer, and innate immunity.
Mauney et al. (2018)
2 more