5awu

X-ray diffraction
2.7Å resolution

Crystal structure of the SGIP1 mu homology domain in complex with an Eps15 fragment containing two DPF motifs (YDPFKGSDPFA)

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-154668 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SH3-containing GRB2-like protein 3-interacting protein 1 Chain: A
Molecule details ›
Chain: A
Length: 282 amino acids
Theoretical weight: 30.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BQI5 (Residues: 552-828; Coverage: 34%)
Gene name: SGIP1
Sequence domains: Muniscin C-terminal mu homology domain
Epidermal growth factor receptor substrate 15 Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.24 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P42566 (Residues: 645-654; Coverage: 1%)
Gene names: AF1P, EPS15

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P4212
Unit cell:
a: 109.562Å b: 109.562Å c: 80.094Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.209 0.245
Expression systems:
  • Escherichia coli
  • Not provided