› 5b16

X-ray diffraction
3.2Å resolution

X-ray structure of DROSHA in complex with the C-terminal tail of DGCR8.

Released:
DOI: 10.2210/pdb5b16/pdb
PDB entry 5b16 coloured by chain, front view.
PDB entry 5b16 coloured by chain, side view.
PDB entry 5b16 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of Human DROSHA.
Kwon SC, Nguyen TA, Choi YG, Jo MH, Hohng S, Kim VN, Woo JS
Cell 164 81-90 (2016)
PMID: 26748718

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186805 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ribonuclease 3 Chain: A
Molecule details ›
Chain: A
Length: 986 amino acids
Theoretical weight: 108.69 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9NRR4 (Residues: 411-1365; Coverage: 70%)
Gene names: DROSHA, RN3, RNASE3L, RNASEN
Sequence domains:
Microprocessor complex subunit DGCR8 Chains: B, C
Molecule details ›
Chains: B, C
Length: 39 amino acids
Theoretical weight: 4.59 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8WYQ5 (Residues: 728-750; Coverage: 3%)
Gene names: C22orf12, DGCR8, DGCRK6, LP4941

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: C2
Unit cell:
a: 117.254Å b: 118.136Å c: 122.296Å
α: 90° β: 102.07° γ: 90°
R-values:
R R work R free
0.267 0.267 0.3
Expression system: Homo sapiens

Quick links

Citations

34 review citations

Regulation of microRNA biogenesis and its crosstalk with other cellular pathways.
Treiber et al. (2019)
33 more

10 mentions without citation

A Glimpse of "Dicer Biology" Through the Structural and Functional Perspective.
Paturi et al. (2021)
9 more