› 5b25

X-ray diffraction
1.9Å resolution

Function and Biology Details

Reaction catalysed: 
Nucleoside 3',5'-cyclic phosphate + H(2)O = nucleoside 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169511 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 338 amino acids
Theoretical weight: 38.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q01064 (Residues: 146-506; Coverage: 63%)
Gene names: PDE1B, PDES1B
Sequence domains: 3'5'-cyclic nucleotide phosphodiesterase
Structure domains: 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain

Ligands and Environments

5 bound ligands:
Ligand ZN 4 x ZN
Ligand MG 4 x MG
Ligand 4QJ 4 x 4QJ
Ligand SO4 10 x SO4
Ligand GOL 8 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P43212
Unit cell:
a: 102.875Å b: 102.875Å c: 294.848Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.172 0.203
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Therapeutic targeting of 3',5'-cyclic nucleotide phosphodiesterases: inhibition and beyond.
Baillie et al. (2019)
6 more

2 mentions without citation

Improved Virus Isoelectric Point Estimation by Exclusion of Known and Predicted Genome-Binding Regions.
Heffron et al. (2020)
1 more