› 5b3z

X-ray diffraction
2.3Å resolution

Crystal structure of hPin1 WW domain (5-39) fused with maltose-binding protein

Released:
DOI: 10.2210/pdb5b3z/pdb
PDB entry 5b3z coloured by chain, front view.
PDB entry 5b3z coloured by chain, side view.
PDB entry 5b3z coloured by chain, top view.
Source organisms:
Primary publication:
Structural studies of the N-terminal fragments of the WW domain: Insights into co-translational folding of a beta-sheet protein.
Hanazono Y, Takeda K, Miki K
Sci Rep 6 34654 (2016)
PMID: 27698466

Function and Biology Details

Reaction catalysed: 
Peptidylproline (omega=180) = peptidylproline (omega=0)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142565 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 403 amino acids
Theoretical weight: 44.64 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: Q13526 (Residues: 5-39; Coverage: 22%)
  • Canonical: P0AEX9 (Residues: 27-393; Coverage: 99%)
Gene names: JW3994, PIN1, b4034, malE
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P21
Unit cell:
a: 84.514Å b: 120.433Å c: 110.67Å
α: 90° β: 97.79° γ: 90°
R-values:
R R work R free
0.183 0.181 0.224
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Cotranslational Folding of Proteins on the Ribosome.
Liutkute et al. (2020)
4 other articles

1 mention without citation

Structural studies of the N-terminal fragments of the WW domain: Insights into co-translational folding of a beta-sheet protein.
Hanazono et al. (2016)