› 5b4o

X-ray diffraction
1.37Å resolution

Crystal structure of Macrophage Migration Inhibitory Factor in complex with BTZO-14

Released:
DOI: 10.2210/pdb5b4o/pdb
PDB entry 5b4o coloured by chain, front view.
PDB entry 5b4o coloured by chain, side view.
PDB entry 5b4o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
BTZO-1, a cardioprotective agent, reveals that macrophage migration inhibitory factor regulates ARE-mediated gene expression.
Kimura H, Sato Y, Tajima Y, Suzuki H, Yukitake H, Imaeda T, Kajino M, Oki H, Takizawa M, Tanida S
Chem Biol 17 1282-94 (2010)
PMID: 21168764

Function and Biology Details

Reactions catalysed: 
Keto-phenylpyruvate = enol-phenylpyruvate
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146863 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage migration inhibitory factor Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 114 amino acids
Theoretical weight: 12.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14174 (Residues: 2-115; Coverage: 99%)
Gene names: GLIF, MIF, MMIF
Sequence domains: Macrophage migration inhibitory factor (MIF)
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

3 bound ligands:
Ligand 6DQ 4 x 6DQ
Ligand EDO 1 x EDO
Ligand SO4 9 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P3121
Unit cell:
a: 95.194Å b: 95.194Å c: 103.296Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.183 0.198
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Review article: the role of oxidative stress in pathogenesis and treatment of inflammatory bowel diseases.
Piechota-Polanczyk et al. (2014)
2 more