› 5b71

X-ray diffraction
2.11Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235009 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
SKY59 Fab light chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 217 amino acids
Theoretical weight: 23.33 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
SKY59 Fab heavy chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 228 amino acids
Theoretical weight: 24.12 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Complement C5 beta chain Chains: E, F
Molecule details ›
Chains: E, F
Length: 110 amino acids
Theoretical weight: 12.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01031 (Residues: 20-124; Coverage: 6%)
Gene names: C5, CPAMD4
Sequence domains: Macroglobulin domain MG1
Structure domains: Immunoglobulin-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: P1
Unit cell:
a: 39.79Å b: 55.1Å c: 127.76Å
α: 89.18° β: 86.24° γ: 78.2°
R-values:
R R work R free
0.248 0.246 0.285
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

25 review citations

Next generation antibody drugs: pursuit of the 'high-hanging fruit'.
Carter et al. (2018)
24 more

4 mentions without citation

Long lasting neutralization of C5 by SKY59, a novel recycling antibody, is a potential therapy for complement-mediated diseases.
Fukuzawa et al. (2017)
3 more