› 5bo4

X-ray diffraction
2.9Å resolution

Structure of SOCS2:Elongin C:Elongin B from DMSO-treated crystals

Released:
DOI: 10.2210/pdb5bo4/pdb
PDB entry 5bo4 coloured by chain, front view.
PDB entry 5bo4 coloured by chain, side view.
PDB entry 5bo4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Serendipitous SAD Solution for DMSO-Soaked SOCS2-ElonginC-ElonginB Crystals Using Covalently Incorporated Dimethylarsenic: Insights into Substrate Receptor Conformational Flexibility in Cullin RING Ligases.
Gadd MS, Bulatov E, Ciulli A
PLoS One 10 e0131218 (2015)
PMID: 26121586

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-126990 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Suppressor of cytokine signaling 2 Chains: A, D, G, J, M, P
Molecule details ›
Chains: A, D, G, J, M, P
Length: 169 amino acids
Theoretical weight: 19.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14508 (Residues: 32-198; Coverage: 84%)
Gene names: CIS2, SOCS2, SSI2, STATI2
Sequence domains:
Structure domains:
Elongin-B Chains: B, E, H, K, N, Q
Molecule details ›
Chains: B, E, H, K, N, Q
Length: 104 amino acids
Theoretical weight: 11.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15370 (Residues: 1-104; Coverage: 88%)
Gene names: ELOB, TCEB2
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Elongin-C Chains: C, F, I, L, O, R
Molecule details ›
Chains: C, F, I, L, O, R
Length: 97 amino acids
Theoretical weight: 10.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15369 (Residues: 17-112; Coverage: 86%)
Gene names: ELOC, TCEB1
Sequence domains: Skp1 family, tetramerisation domain
Structure domains: Potassium Channel Kv1.1; Chain A

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CAS 12 x CAS

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P32
Unit cell:
a: 185.217Å b: 185.217Å c: 67.204Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.229 0.287
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Regulation of Macrophage, Dendritic Cell, and Microglial Phenotype and Function by the SOCS Proteins.
McCormick et al. (2015)
4 more

3 mentions without citation

Serendipitous SAD Solution for DMSO-Soaked SOCS2-ElonginC-ElonginB Crystals Using Covalently Incorporated Dimethylarsenic: Insights into Substrate Receptor Conformational Flexibility in Cullin RING Ligases.
Gadd et al. (2015)
2 more