› 5bro

X-ray diffraction
2.4Å resolution

Crystal structure of modified HexB (modB)

Released:
DOI: 10.2210/pdb5bro/pdb
PDB entry 5bro coloured by chain, front view.
PDB entry 5bro coloured by chain, side view.
PDB entry 5bro coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Protease-resistant modified human β-hexosaminidase B ameliorates symptoms in GM2 gangliosidosis model.
Kitakaze K, Mizutani Y, Sugiyama E, Tasaki C, Tsuji D, Maita N, Hirokawa T, Asanuma D, Kamiya M, Sato K, Setou M, Urano Y, Togawa T, Otaka A, Sakuraba H, Itoh K
J Clin Invest 126 1691-703 (2016)
PMID: 27018595

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139695 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-hexosaminidase subunit beta Chain: A
Molecule details ›
Chain: A
Length: 514 amino acids
Theoretical weight: 58.74 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P07686 (Residues: 43-556; Coverage: 100%)
Gene names: HCC7, HEXB
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand GOL 1 x GOL
Ligand FMT 1 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P42212
Unit cell:
a: 126.47Å b: 126.47Å c: 88.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.187 0.24
Expression system: Cricetulus griseus

Quick links

Citations

2 review citations

Mass Spectrometry Imaging for Glycome in the Brain.
Hasan et al. (2021)
1 more

4 mentions without citation

Protease-resistant modified human β-hexosaminidase B ameliorates symptoms in GM2 gangliosidosis model.
Kitakaze et al. (2016)
3 more