› 5bt1

X-ray diffraction
2.62Å resolution

histone chaperone Hif1 playing with histone H2A-H2B dimer

Released:
DOI: 10.2210/pdb5bt1/pdb
PDB entry 5bt1 coloured by chain, front view.
PDB entry 5bt1 coloured by chain, side view.
PDB entry 5bt1 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural Insights into the Association of Hif1 with Histones H2A-H2B Dimer and H3-H4 Tetramer.
Zhang M, Liu H, Gao Y, Zhu Z, Chen Z, Zheng P, Xue L, Li J, Teng M, Niu L
Structure 24 1810-1820 (2016)
PMID: 27618665

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-135449 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HAT1-interacting factor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 393 amino acids
Theoretical weight: 44.55 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12373 (Residues: 1-385; Coverage: 100%)
Gene names: HIF1, L1205, YLL022C
Structure domains: Tetratricopeptide repeat domain
Histone H2A.1 Chain: C
Molecule details ›
Chain: C
Length: 143 amino acids
Theoretical weight: 15.31 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P04911 (Residues: 1-132; Coverage: 100%)
Gene names: H2A1, HTA1, SPT11, YD9934.10, YDR225W
Sequence domains:
Structure domains: Histone, subunit A
Histone H2B.1 Chain: D
Molecule details ›
Chain: D
Length: 142 amino acids
Theoretical weight: 15.58 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P02293 (Residues: 1-131; Coverage: 100%)
Gene names: H2B1, HTB1, SPT12, YD9934.09C, YDR224C
Sequence domains: Core histone H2A/H2B/H3/H4
Structure domains: Histone, subunit A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 102.673Å b: 48.363Å c: 160.706Å
α: 90° β: 107.05° γ: 90°
R-values:
R R work R free
0.21 0.208 0.262
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Epigenetic signatures in cardiac fibrosis, special emphasis on DNA methylation and histone modification.
Tao et al. (2018)
2 more

1 mention without citation

Molecular basis for chromatin assembly and modification by multiprotein complexes.
Ricketts et al. (2019)