› 5bv8

X-ray diffraction
1.59Å resolution

G1324S mutation in von Willebrand Factor A1 domain

Released:
DOI: 10.2210/pdb5bv8/pdb
PDB entry 5bv8 coloured by chain, front view.
PDB entry 5bv8 coloured by chain, side view.
PDB entry 5bv8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mutational Constraints on Local Unfolding Inhibit the Rheological Adaptation of von Willebrand Factor.
Tischer A, Campbell JC, Machha VR, Moon-Tasson L, Benson LM, Sankaran B, Kim C, Auton M
J Biol Chem 291 3848-59 (2016)
PMID: 26677223

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137563 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
von Willebrand factor Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 28.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04275 (Residues: 1238-1471; Coverage: 8%)
Gene names: F8VWF, VWF
Sequence domains:
Structure domains: von Willebrand factor, type A domain

Ligands and Environments

2 bound ligands:
Ligand EDO 1 x EDO
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P61
Unit cell:
a: 86.45Å b: 86.45Å c: 68.16Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.164 0.163 0.181
Expression system: Escherichia coli

Quick links

Citations

2 review citations

A biophysical view on von Willebrand factor activation.
Löf et al. (2018)
1 more

4 mentions without citation

Mutational Constraints on Local Unfolding Inhibit the Rheological Adaptation of von Willebrand Factor.
Tischer et al. (2016)
3 more