› 5bw7

X-ray diffraction
3Å resolution

Crystal structure of nonfucosylated Fc Y296W mutant complexed with bis-glycosylated soluble form of Fc gamma receptor IIIa

Released:
DOI: 10.2210/pdb5bw7/pdb
PDB entry 5bw7 coloured by chain, front view.
PDB entry 5bw7 coloured by chain, side view.
PDB entry 5bw7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Importance of the Side Chain at Position 296 of Antibody Fc in Interactions with FcγRIIIa and Other Fcγ Receptors.
Isoda Y, Yagi H, Satoh T, Shibata-Koyama M, Masuda K, Satoh M, Kato K, Iida S
PLoS One 10 e0140120 (2015)
PMID: 26444434

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-134849 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (6 distinct):
Immunoglobulin heavy constant gamma 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 223 amino acids
Theoretical weight: 25.12 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P01857 (Residues: 108-328; Coverage: 55%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Low affinity immunoglobulin gamma Fc region receptor III-A Chain: C
Molecule details ›
Chain: C
Length: 179 amino acids
Theoretical weight: 20.64 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P08637 (Residues: 21-193; Coverage: 73%)
Gene names: CD16A, FCG3, FCGR3, FCGR3A, IGFR3
Sequence domains: Immunoglobulin domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P41212
Unit cell:
a: 77.273Å b: 77.273Å c: 350.983Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.25
Expression system: Cricetulus griseus

Quick links

Citations

4 review citations

A synopsis of recent developments defining how N-glycosylation impacts immunoglobulin G structure and function.
Yamaguchi et al. (2020)
3 more

3 mentions without citation

Assessing the Heterogeneity of the Fc-Glycan of a Therapeutic Antibody Using an engineered FcγReceptor IIIa-Immobilized Column.
Kiyoshi et al. (2018)
2 more