› 5byy

X-ray diffraction
2.79Å resolution

ERK5 IN COMPLEX WITH SMALL MOLECULE

Released:
DOI: 10.2210/pdb5byy/pdb
PDB entry 5byy coloured by chain, front view.
PDB entry 5byy coloured by chain, side view.
PDB entry 5byy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Discovery of a novel allosteric inhibitor-binding site in ERK5: comparison with the canonical kinase hinge ATP-binding site.
Chen H, Tucker J, Wang X, Gavine PR, Phillips C, Augustin MA, Schreiner P, Steinbacher S, Preston M, Ogg D
Acta Crystallogr D Struct Biol 72 682-93 (2016)
PMID: 27139631

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171583 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase 7 Chain: A
Molecule details ›
Chain: A
Length: 346 amino acids
Theoretical weight: 39.61 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q13164 (Residues: 49-394; Coverage: 42%)
Gene names: BMK1, ERK5, MAPK7, PRKM7
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 4WG 1 x 4WG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P41212
Unit cell:
a: 92.791Å b: 92.791Å c: 107.321Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.221 0.273
Expression system: Spodoptera frugiperda

Quick links

Citations

1 review citation

Small molecule ERK5 kinase inhibitors paradoxically activate ERK5 signalling: be careful what you wish for….
Cook et al. (2020)
4 other articles

5 mentions without citation

Identification and characterization of fragment binding sites for allosteric ligand design using the site identification by ligand competitive saturation hotspots approach (SILCS-Hotspots).
MacKerell et al. (2020)
4 more