› 5c0r

X-ray diffraction
3.19Å resolution

Crystal Structure of a Generation 3 Influenza Hemagglutinin Stabilized Stem Complexed with the Broadly Neutralizing Antibody C179

Released:
DOI: 10.2210/pdb5c0r/pdb
PDB entry 5c0r coloured by chain, front view.
PDB entry 5c0r coloured by chain, side view.
PDB entry 5c0r coloured by chain, top view.
Source organisms:
Primary publication:
Hemagglutinin-stem nanoparticles generate heterosubtypic influenza protection.
Yassine HM, Boyington JC, McTamney PM, Wei CJ, Kanekiyo M, Kong WP, Gallagher JR, Wang L, Zhang Y, Joyce MG, Lingwood D, Moin SM, Andersen H, Okuno Y, Rao SS, Harris AK, Kwong PD, Mascola JR, Nabel GJ, Graham BS
Nat Med 21 1065-70 (2015)
PMID: 26301691

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229695 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Fibritin C-terminal domain-containing protein; Hemagglutinin HA2 chain; Transmembrane protein gp41 Chain: A
Molecule details ›
Chain: A
Length: 305 amino acids
Theoretical weight: 34.42 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: Q6WG00 (Residues: 328-402, 403-405, 436-517; Coverage: 28%)
  • Canonical: P04578 (Residues: 546-577; Coverage: 4%)
  • Canonical: D9IEJ2 (Residues: 458-485; Coverage: 6%)
Gene names: HA, T4Tp161, env, wac
Sequence domains: Haemagglutinin
C179 Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.45 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
C179 Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 233 amino acids
Theoretical weight: 25.06 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: R3
Unit cell:
a: 112.038Å b: 112.038Å c: 205.125Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.199 0.251
Expression systems:
  • Homo sapiens
  • Mus musculus

Quick links

Citations

135 review citations

The human antibody response to influenza A virus infection and vaccination.
Krammer F. (2019)
134 more

1 mention without citation

α/β coiled coils.
Hartmann et al. (2016)