› 5c0s

X-ray diffraction
4.3Å resolution

Crystal structure of a generation 4 influenza hemagglutinin stabilized stem in complex with the broadly neutralizing antibody CR6261

Released:
DOI: 10.2210/pdb5c0s/pdb
PDB entry 5c0s coloured by chain, front view.
PDB entry 5c0s coloured by chain, side view.
PDB entry 5c0s coloured by chain, top view.
Source organisms:
Primary publication:
Hemagglutinin-stem nanoparticles generate heterosubtypic influenza protection.
Yassine HM, Boyington JC, McTamney PM, Wei CJ, Kanekiyo M, Kong WP, Gallagher JR, Wang L, Zhang Y, Joyce MG, Lingwood D, Moin SM, Andersen H, Okuno Y, Rao SS, Harris AK, Kwong PD, Mascola JR, Nabel GJ, Graham BS
Nat Med 21 1065-70 (2015)
PMID: 26301691

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229696 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fibritin C-terminal domain-containing protein; Hemagglutinin HA2 chain; Transmembrane protein gp41 Chain: A
Molecule details ›
Chain: A
Length: 305 amino acids
Theoretical weight: 34.21 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: Q6WG00 (Residues: 328-402, 403-405, 436-517; Coverage: 28%)
  • Canonical: P04578 (Residues: 546-577; Coverage: 4%)
  • Canonical: D9IEJ2 (Residues: 458-485; Coverage: 6%)
Gene names: HA, T4Tp161, env, wac
Sequence domains: Haemagglutinin
CR6261 antibody heavy chain Chain: H
Molecule details ›
Chain: H
Length: 226 amino acids
Theoretical weight: 23.98 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
CR6261 antibody light chain Chain: L
Molecule details ›
Chain: L
Length: 221 amino acids
Theoretical weight: 23.26 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: R3
Unit cell:
a: 101.649Å b: 101.649Å c: 186.022Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.241 0.238 0.31
Expression system: Homo sapiens

Quick links

Citations

134 review citations

The human antibody response to influenza A virus infection and vaccination.
Krammer F. (2019)
133 more

4 mentions without citation

Structural insights into the design of novel anti-influenza therapies.
Wu et al. (2018)
3 more