› 5c4m

X-ray diffraction
1.3Å resolution

RhoA GDP with novel switch II conformation

Released:
DOI: 10.2210/pdb5c4m/pdb
PDB entry 5c4m coloured by chain, front view.
PDB entry 5c4m coloured by chain, side view.
PDB entry 5c4m coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Pellegrini E, Bowler MW

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158275 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transforming protein RhoA Chain: A
Molecule details ›
Chain: A
Length: 193 amino acids
Theoretical weight: 21.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61586 (Residues: 1-193; Coverage: 100%)
Gene names: ARH12, ARHA, RHO12, RHOA
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 31.796Å b: 66.271Å c: 83.679Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.17 0.2
Expression system: Escherichia coli

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