Function and Biology Details
Reactions catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140112 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase ICP0
Molecule details ›
Chain: B
Length: 21 amino acids
Theoretical weight: 2.23 KDa
Source organism: Human alphaherpesvirus 1
Expression system: Not provided
UniProt:
Length: 21 amino acids
Theoretical weight: 2.23 KDa
Source organism: Human alphaherpesvirus 1
Expression system: Not provided
UniProt:
- Canonical:
P08393 (Residues: 613-633; Coverage: 3%)
Ubiquitin carboxyl-terminal hydrolase 7
Molecule details ›
Chain: A
Length: 548 amino acids
Theoretical weight: 64.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 548 amino acids
Theoretical weight: 64.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: Q93009 (Residues: 560-1102; Coverage: 49%)
Sequence domains:
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
SSRF BEAMLINE BL17U
Spacegroup:
P212121
Expression systems:
- Not provided
- Escherichia coli BL21(DE3)
