› 5c67

X-ray diffraction
1.83Å resolution

Human Mesotrypsin in complex with amyloid precursor protein inhibitor variant APPI-M17G/I18F/F34V

Released:
DOI: 10.2210/pdb5c67/pdb
PDB entry 5c67 coloured by chain, front view.
PDB entry 5c67 coloured by chain, side view.
PDB entry 5c67 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Combinatorial protein engineering of proteolytically resistant mesotrypsin inhibitors as candidates for cancer therapy.
Cohen I, Kayode O, Hockla A, Sankaran B, Radisky DC, Radisky ES, Papo N
Biochem J 473 1329-41 (2016)
PMID: 26957636

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138360 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Trypsin-3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 224 amino acids
Theoretical weight: 24.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P35030 (Residues: 81-304; Coverage: 74%)
Gene names: PRSS3, PRSS4, TRY3, TRY4
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Soluble APP-beta Chains: C, E
Molecule details ›
Chains: C, E
Length: 81 amino acids
Theoretical weight: 9.13 KDa
Source organism: Homo sapiens
Expression system: Komagataella phaffii GS115
UniProt:
  • Canonical: P05067 (Residues: 294-346; Coverage: 7%)
Gene names: A4, AD1, APP
Sequence domains: Kunitz/Bovine pancreatic trypsin inhibitor domain
Structure domains: Pancreatic trypsin inhibitor Kunitz domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P41212
Unit cell:
a: 78.15Å b: 78.15Å c: 243.73Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.223 0.267
Expression systems:
  • Escherichia coli BL21
  • Komagataella phaffii GS115

Quick links

Citations

3 review citations

Surface loops of trypsin-like serine proteases as determinants of function.
Goettig et al. (2019)
2 more

6 mentions without citation

Miniproteins as a Powerful Modality in Drug Development.
Crook et al. (2020)
5 more